Solutions:- Field Stain A and B, Preparation of the solution
updated: June 5, 2023 by kamlesh kumar
Field Stain A And B
Field stain consists of:
- Field stain A
- Field stain B
Thin and thick blood films are made and fixed in methanol for one minute.
Field Stain A (Dark violet color)
Using Commercially Available Powder.
- Field stain A commercially available powder = 5 grams
- Distilled water is heated to 80 °C or kept at 60 °C for 30 minutes. = 600 mL.
Procedure to make Field stan A from commercial powder:
Field stain A (ready-made powder).
- Mix the powder into the water until it is dissolved.
- Filter the solution.
- Label the date.
Field stain A from the reagents:
- Methylene blue (analytical grade) = 1.6 grams.
- Azur = 1 gram.
- Disodium dihydrogen phosphate anhydrous = 10 grams.
- Potassium dihydrogen phosphate anhydrous = 12.5 grams.
- Distilled water = 1000 mL.
Procedure For Field Stain A Preparation:
- Take Methylene blue (analytical grade) = 1.6 grams.
- Take Azur = 1 gram.
- Take Disodium dihydrogen phosphate anhydrous = 10 grams.
- Take Potassium dihydrogen phosphate anhydrous = 12.5 grams.
- Distilled water = 1000 mL.
- Mix both salts (3 and 4) in the water.
- Add 500 ml from above into a bottle containing glass beads.
- Add the stain powders (Methylene blue and Azur) and mix well.
- Then add the remainder of the solution.
- Mix well and filter into another clean bottle.
- Label the bottle Field stain A and also write the date.
Field Stain B (Orange color)
Using Commercially Available Powder:
- Field stain B commercially available powder = 4.8 grams.
- Distilled water is heated to 80 °C or kept at 60 °C for 30 min. = 600 mL.
- Mix well the field stain B until it is dissolved.
- Filter and label the date.
Field stain B reagents:
- Eosin(yellow water soluble) powder = 2.0 grams.
- Disodium dihydrogen phosphate anhydrous = 10 grams.
- Potassium dihydrogen phosphate anhydrous = 12.5 grams.
- Distilled water = 1000 mL.
Procedure for Field stain B preparation:
- Mix both salts (2 and 3) in the distle water (1000 mL) to make the solution.
- Add 500 ml from above into a bottle containing glass beads.
- Add the stain powders Eosin and mix well.
- Then add the remainder of the solution.
- Mix well and filter into another clean bottle.
- Label the bottle Field stain B, and also write the date.
Staining Procedure for Field Stain A and B:
- Fill up two Coplin jars or wide-mouth bottles:
- Field Stain A (Blue stain).
- Field Stain B (Red stain).
- Make a blood smear on a clean glass slide, and it is dried in the air.
- Fix in methanol for one minute or get Spray ‘Easyfix’.
- Dry in the air.
- Dip fixed smear to Field Stain B (Red Stain) for 5 to 6 seconds.
- Wash in running tap water.
- Dip smear into Field Stain A (Blue Stain) for 10 to 30 seconds (adjust it).
- Wash in running tap water.
- Dry at air and see under oil immersion objective.
- The staining time may be adjusted.
![](https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEg6PfkQngpxph2P8qt8jrWHq74lfUJ-bdVkvIt_Lfe3VcSni59M6X9GY_R7geM4zUzchQ9iHVscFVWwuFSQ-wfmevbj44UG6JAdDVndWtneEs2e24ny1Msz82vK-lp5qoMAgms7kmT31wEaLsMPpKD5drR7XW3EDXoHN60h1b-B2Pw6r0iUK7ugHX7F/w640-h398/Field%20stain%201,1.png.webp)
Field stain procedure
Interpretation of the cells in Field stain:
![](https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEhLgeUgKtVhGacxY3I02xHaLvXixpLlhef9EAiqu1s8nxxWN0csTuL8WlmMoSf_89VkcFAx5xGnDYGrSjIzD4R0_ZAXUx1TcFIs3TFLj-S_pBqHQrHWuG27k7ubQZwszW4xRy5M8zYlGPHfu9rIRqa5SE0MnMJcwidRy776DcpjfopHlw4KQRjNLH6Z/w640-h408/Field%20stain%201,2.png.webp)
Field stain smear
How to prepare thick and thin smears:
- Thick and thin blood smears can be made into two separate slides.
- Put two separate drops of the blood. For a thin smear, put a small drop and a large drop for a thick smear.
- Spread the drops by the cover glass.
![](https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgAGOXQmli2le9AcTykH2p0liG-AbcPEgU_R-26uI7RfSL5-mZqYUl2dw_I0IICioVibGOA8yO8UYIe4O-wgxUI1Xntb6nWk7PNeGJyrb5U-zbqr_-Oy1Y48pPRgUGAD_NpE9hEdbGzAi-MbqbwFtiJdqsUS0A8fjjDDEiBCTTdhwZBz2RVxP8qLbwX/w612-h640/Field%20stain%201,3.png.webp)
How to make blood smear thin and thick
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